Journal: Frontiers in Immunology
Article Title: Zika virus induces monocyte recruitment in the immunocompetent adult brain driving chronic inflammation
doi: 10.3389/fimmu.2025.1597776
Figure Lengend Snippet: Infiltrating monocytes seen in ZIKV mice brains at 7dpi. (A) CCR2-CreER;R26R-EGFP (Ai6) mice were given tamoxifen for 3 days before infected with ZIKV and harvested. (B) Quantification of flow data for Bone marrow cells (CCR2-EGFP+, CCR2-EGFP+ CD68+) (C) Quantification of flow data for Brain cells (CD11B+CD45+, CD45int CD11B+ (Microglia), CCR2-EGFP+, CCR2-EGFP-). (D, E) Immunofluorescence of CCR2-CreER-R26R-EGFP mice for EGFP and sall1 expression in choroid plexus region with quantification. N=3-4. Data represents the mean ± SD ∗ p < 0.0332, ∗∗ p < 0.0021, and ∗∗∗ p < 0.0002.
Article Snippet: Primary antibodies used were SALL1 (eBioscience), and GFAP (sc33673), and secondary antibodies used were AF647 (Invitrogen).
Techniques: Infection, Immunofluorescence, Expressing